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本实验制备组成分别为DOPE/Chol/OA(4:4:3)的酸敏脂质体及DOPC/Chol/OA(4:4:3)脂质体,用于包裹质粒pSV_2-neo、pUC18-ras、pSV-neo-ras及大分子线状DNA,包裹率可达50％.被脂质体包裹的DNA不被DNase降解,提示DNA分子被脂质体包裹在微球体内部.电泳检测表明,被包裹的DNA分子没有断裂.所得到的脂质体较稳定,4℃放置5～6月,脂质体内仍保留部分DNA分子.制备酸敏脂质体时,pH为8.0时脂质体较稳定,pH<6.5～7时则不稳定.
In this experiment, acid-sensitive liposomes with DOPE / Chol / OA (4: 4: 3) and DOPC / Chol / OA (4: 4: 3) liposomes were prepared and used to encapsulate plasmids pSV_2-neo, pUC18 -ras, pSV-neo-ras and macromolecular linear DNA, the encapsulation efficiency can reach 50% .The liposome-encapsulated DNA is not degraded by DNase, which indicates that the DNA molecules are encapsulated by liposomes in the microspheres.The results of electrophoresis , The wrapped DNA molecules are not broken.The resulting liposomes are more stable, placed at 4 ℃ for 5 ~ 6 months, liposomes still retain some DNA molecules.Preparation of acid-sensitive liposomes, the pH of 8.0 liposomes More stable, pH <6.5 ~ 7 instability.