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为研究单核细胞趋化蛋白 - 1对单核细胞表面淋巴细胞功能相关抗原 - 1、清道夫受体和载脂蛋白E表达的影响 ,采用培养人单核细胞株THP - 1细胞 ,以间接免疫荧光法结合流式细胞术和激光共聚焦扫描显微镜分别检测淋巴细胞功能相关抗原 - 1和清道夫受体蛋白的表达 ,用逆转录 -多聚酶链反应检测清道夫受体和载脂蛋白EmRNA的表达水平。结果显示 ,在单核细胞趋化蛋白 - 1刺激组淋巴细胞功能相关抗原 - 1和清道夫受体蛋白的表达明显高于对照组 (P <0 .0 1) ;17- β -雌二醇可部分抑制单核细胞趋化蛋白 - 1对THP - 1细胞表面淋巴细胞功能相关抗原 - 1表达的促进作用 (P <0 .0 5 ) ,但对单核细胞趋化蛋白 - 1促进清道夫受体表达的作用无明显影响 ;单核细胞趋化蛋白 - 1明显促进THP - 1细胞清道夫受体mRNA的表达 ,而对载脂蛋白EmRNA的表达无明显影响。研究提示 ,单核细胞趋化蛋白 - 1可通过促进单核细胞表面粘附分子、淋巴细胞功能相关抗原 - 1以及清道夫受体的表达而促进动脉粥样硬化的发生 ,雌二醇还可能通过抑制单核细胞趋化蛋白 - 1对淋巴细胞功能相关抗原- 1表达的促进而发挥抗动脉粥样硬化作用。
To investigate the effect of monocyte chemoattractant protein - 1 on monocyte surface lymphocyte function - related antigen - 1, scavenger receptor and apolipoprotein E expression, human monocytic THP - 1 cells were cultured in order to indirectly Immunofluorescence combined with flow cytometry and confocal laser scanning microscopy were used to detect the expression of lymphocyte function-related antigen-1 and scavenger receptor protein, respectively. The expression of scavenger receptor and apolipoprotein EmRNA was detected by reverse transcription polymerase chain reaction The expression level. The results showed that the expression of lymphocyte function - associated antigen - 1 and scavenger receptor protein in monocyte chemoattractant protein - 1 stimulated group was significantly higher than that in the control group (P <0.01); 17 - beta - estradiol Could partially inhibit the promotion of monocyte chemoattractant protein - 1 on the expression of functional lymphocyte - associated antigen - 1 on THP - 1 cells (P <0.05), but not on monocyte chemoattractant protein - 1 The expression of scavenger receptor mRNA in THP - 1 cells was significantly enhanced by monocyte chemoattractant protein - 1, but had no significant effect on the expression of apolipoprotein EmRNA. Studies suggest that monocyte chemotactic protein - 1 may promote atherosclerosis by promoting the expression of monocyte adhesion molecules, lymphocyte function - associated antigen - 1 and scavenger receptors, and estradiol may also Inhibits atherosclerosis by inhibiting the promotion of monocyte chemotactic protein - 1 on lymphocyte function - associated antigen - 1 expression.