论文部分内容阅读
目的:探讨倒千里光碱的胚胎发育毒性。方法:采用小鼠全胚胎培养模型,将8.5 d小鼠胚胎在含有倒千里光碱的即刻离心血清中培养48 h(倒千里光碱的终浓度分别为12.5,25,50,100 mg.L-1),观察倒千里光碱对胚胎生长发育(卵黄囊直径、颅臀长、头长、体节数)和组织器官形态分化(卵黄囊循环、尿囊、翻转、心、脑、尾神经管、视听嗅系统、腮弓、颌突、肢芽)的影响。结果:倒千里光碱对胚胎的生长发育和形态分化均具有明显的毒性影响。随倒千里光碱浓度的增加,胚胎的生长发育和组织器官形态分化指标影响越来越严重,其中听嗅系统、腮弓、上下颌突、前后肢芽的影响最为明显。结论:倒千里光碱对体外培养的小鼠胚胎有明显的毒性作用,提示妊娠期暴露于该化合物对胎儿具有潜在的毒性。
Objective: To investigate the embryonic developmental toxicity of Trichostatin. METHODS: Mouse embryonic whole-embryo cultures were used to culture 8.5-day-old mouse embryos in immediate-centrifugation sera containing serylriperitone for 48 h (final concentrations of trichlorethylene were 12.5, 25, 50, 100 mg.L-1 ), Observe the effects of JXL on embryonic growth and development (the diameter of yolk sac, the length of cranial buttocks, the length of head, the number of body segments) and the differentiation of tissues and organs (yolk sac circulation, allantoic sac, Audio-visual snoring system, the arch bow, jaw protrusion, limb bud). Results: Trillium alkaloids had obvious toxic effects on embryonic growth and morphological differentiation. With the increase of the concentration of Trilithium, the growth and development of embryos and the morphological differentiation of tissues and organs have more and more serious effects. Among them, the auditory and olfactory system, the maxillary and mandibular processes, the anterior and posterior limb buds have the most obvious effects. CONCLUSION: Pfizer’s alkaloids have obvious toxic effects on mouse embryos cultured in vitro, suggesting that exposure to this compound during pregnancy may be potentially toxic to the fetus.