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以海豚链球菌Streptococcus iniae灭活菌苗为免疫原,通过注射、浸泡、口服3种途径对体重为(100±10)g的奥尼罗非鱼Oreochromis niloticus进行免疫,在免疫前和免疫后的第3、7、10、14、21天对试验鱼进行尾静脉采血,测定其血液中白细胞的数量、各类白细胞的数量及其吞噬活性,以及血清的抗菌活力、溶菌酶活性、超氧化物歧化酶(SOD)活力及抗体水平。在免疫后第22天对所有试验鱼按照1.0×107cfu/尾进行攻毒。结果表明:各免疫组白细胞总数、淋巴细胞、嗜中性粒细胞和单核细胞数量均显著高于对照组(P<0.05);血清的抗菌活力、溶菌酶活性和抗体水平与对照组差异显著(P<0.05),SOD活力和对照组无显著差异(P>0.05);免疫的罗非鱼对海豚链球菌的抵抗力明显增强,其中注射组罗非鱼的免疫能力明显高于浸泡和口服组,加强免疫组的免疫能力均有明显增强趋势。
Immunization of Oreochromis niloticus with the body weight of (100 ± 10) g by injection, immersion and oral administration using Streptococcus iniae inactivated vaccine as immunogen showed that pre-immunization and post-immunization On the 3rd, 7th, 10th, 14th, 21st and 21st days, blood was collected from the tail vein of the test fish to determine the number of white blood cells, the number of leukocytes and their phagocytic activity, the antibacterial activity, lysozyme activity, superoxide Dismutase (SOD) activity and antibody levels. All test fish were challenged at 1.0 × 107 cfu / tail on day 22 post-immunization. The results showed that the total number of leukocytes, lymphocytes, neutrophils and monocytes in each immunized group were significantly higher than those in the control group (P <0.05). The antibacterial activity, lysozyme activity and antibody level in serum were significantly different from those in the control group (P <0.05). There was no significant difference in the activity of SOD between the two groups (P> 0.05). The immunity of tilapia to Streptococcus pulex increased obviously. The immunity of tilapia in the injected group was significantly higher than that of the soaking and orally Group, strengthen the immune group immune capacity have significantly enhanced the trend.