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样品经 HPLC分离后 ,利用氢化可的松对 Ce(IV) - SO-23 化学发光体系的增敏作用 ,在色谱柱为 C18氰基柱 ,水∶乙腈 80∶ 2 0 (体积分数 )为流动相的条件下 ,实现了对氢化可的松的分离与测定 ,方法的线性范围为 4× 1 0 -6～ 1×1 0 -3g/m L,检出限为 9× 1 0 -7g/m L,相对标准偏差 3.6% (n=1 1 )
After the sample was separated by HPLC, the sensitizing effect of hydrocortisone to Ce (IV) -SO-23 chemiluminescence system was measured on a C18 column with a mobile phase of water 80:20 (volume fraction) Phase under the conditions of the separation and determination of hydrocortisone, the linear range of the method 4 × 10-6 ~ 1 × 10-3g / m L, the detection limit of 9 × 10 -7g / m L, relative standard deviation 3.6% (n = 1 1)