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以隶属于44科的60种药用植物为试材,以无水乙醇作为提取溶剂,采用生长速率法和孢子萌发法对选取的药用植物提取物进行抑菌活性的测定,以筛选出对葡萄炭疽病菌具有抑菌活性的药用植物资源。结果表明:在供试质量浓度为100mg/mL时,对葡萄炭疽病菌孢子萌发抑制率和菌丝生长抑制率均在80%以上的有11种植物提取物,抑制率超过90%的药用植物有艾蒿、苍耳、丁香、黄芩、苦参、肉桂和石菖蒲7种,其中丁香、肉桂和石菖蒲的提取物对孢子的萌发抑制率和菌丝生长抑制率均为100%。筛选试验结果表明,药用植物丁香、肉桂和石菖蒲具有开发成防治葡萄炭疽病新型植物源杀菌剂的潜力。
Using 60 kinds of medicinal plants belonging to 44 families as test materials and anhydrous ethanol as extraction solvent, the antibacterial activity of selected medicinal plant extracts was determined by growth rate method and spore germination method, Medicinal plant resources of grape anthracnose bacteriostasis activity. The results showed that there were 11 kinds of plant extracts with inhibitory rate of spore germination of more than 80% and mycelium growth rate of 80% at the tested concentration of 100 mg / mL, and the medicinal plants with the inhibition rate over 90% There are seven kinds of Artemisia, Xanthium, clove, Scutellaria, Sophora, cinnamon and Acorus calamus, of which clove, cinnamon and Acorus calamus germination inhibition rate and mycelium growth inhibition rate was 100%. Screening tests showed that the medicinal plants clove, cinnamon, and Acorus calamus have the potential to develop novel botanical germicide agents against grape anthracnose.