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目的探讨亚慢性砷暴露对小鼠精子发育和调控基因Uty表达影响。方法雄性小鼠随机分为对照组、三氧化二砷(As2O3)1、2、4 mg/L暴露组,每组10只,自然饮用含不同浓度As2O3水染毒,连续暴露60 d。取双侧睾丸、附睾,称重计算脏器系数,显微镜下观察精子活动度,苏木素-伊红染色观察精子畸形率和生精周期各级生精细胞数量,实时定量PCR检测Y染色体Uty基因表达。结果对照组、1、2、4 mg/L染砷组小鼠睾丸脏器系数分别为(3.6±0.41)、(3.47±0.41)、(3.15±0.46)、(3.14±0.45),附睾脏器系数分别为(0.82±0.14)、(0.80±0.12)、(0.70±0.11)、(0.68±0.11),与对照组比较,2、4 mg/L染砷组小鼠睾丸、附睾脏器系数明显降低(P<0.05);2、4 mg/L染砷组小鼠精子活动度分别为82%、81%,明显低于对照组(97%),差异有统计学意义(P<0.05),4 mg/L染砷组小鼠精子畸形率(6.6%)明显高于对照组(2.6%)(P<0.05)。与对照组比较,4 mg/L染砷小鼠睾丸中A型精原细胞、前细线期精母细胞、中粗线期精母细胞、第七阶段精细胞数量[分别为(33.4±3.8)%、(4.0±4.1)%、(125±1.2)%、(220±18.8)%]明显下降(P<0.05);与对照组比较,2、4 mg/L砷染毒小鼠睾丸和附睾内Uty基因表达下降,差异均有统计学意义(P<0.05)。结论亚慢性砷暴露影响小鼠精子发育,其雄性生殖毒性作用可能与Uty基因表达下调有关。
Objective To investigate the effect of subchronic arsenic exposure on sperm development and regulation of Uty expression in mice. Methods Male mice were randomly divided into control group, arsenic trioxide (As2O3) 1, 2, 4 mg / L exposure group, each group of 10, natural drinking water containing different concentrations of As2O3 exposure, continuous exposure 60 d. The testes and epididymis were weighed and the organ coefficient was calculated. The activity of sperm was observed under a microscope. The sperm abnormality rate and spermatogenic cell number at each stage of spermatogenesis were observed by hematoxylin-eosin staining. The expression of Uty gene in Y chromosome was detected by real-time PCR . Results In the control group, the testicular organ coefficients of mice in the arsenic-exposed group were (3.6 ± 0.41), (3.47 ± 0.41), (3.15 ± 0.46), (3.14 ± 0.45), respectively (0.82 ± 0.14), (0.80 ± 0.12), (0.70 ± 0.11) and (0.68 ± 0.11) respectively. Compared with the control group, the organ coefficient of testes and epididymis in 2,4-mg / (P <0.05). The activity of sperm in 2,4-mg / L arsenic-treated mice was 82% and 81%, respectively, which was significantly lower than that in the control group (97% The sperm deformity rate (6.6%) of 4 mg / L arsenic-treated mice was significantly higher than that of control group (2.6%) (P <0.05). Compared with the control group, type A spermatogonia, pre-spermatocyte, mid-spermatocyte, spermatophore in spermatogenic cells in the 4 mg / L arsenic-stained mice and spermatocytes in the seventh stage [(33.4 ± 3.8 ), (4.0 ± 4.1)%, (125 ± 1.2)%, (220 ± 18.8)%] significantly decreased compared with the control group Uty gene expression in the epididymis decreased, the difference was statistically significant (P <0.05). Conclusion Subchronic arsenic exposure may affect sperm development in mice and its male reproductive toxicity may be related to the down regulation of Uty gene expression.