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采用种子乳液聚合方法制得了微米尺度的高磁性物质含量的磁性复合微球.聚合体系中,以0.7～0.8μm的Fe3O4磁性聚集体细乳液作为种子,将苯乙烯,二乙烯基苯作为聚合单体加入到磁性聚集体细乳液中,对Fe3O4磁性聚集体进行溶胀后进行聚合.研究了聚合过程中,溶胀时间对复合微球形貌和磁性物质含量的影响,获得系列形貌微球.通过透射电镜(TEM)、热重分析(TGA)、红外分析(FTIR)、振动样品磁强计(VSM)等表征手段对所制备的磁性聚合物微球进行分析表征.结果显示,所得到的磁性聚合物微球粒度为0.7～1μm,尺寸分布较均一,具有超顺磁性,磁性物质含量为29wt%～57wt%.然后又通过丙烯酸和苯乙烯共聚对微球表面羧基功能化后,得到了表面羧基密度为0.92mmol/g的微球,再将所制备的微球与生物分子偶联后(以hCG作为模式待检分子),在化学发光免疫检测上进行了初步的应用,取得到了较好的应用结果.
Magnetic microspheres with high magnetic material content on the microscale were prepared by seed emulsion polymerization.The microspheres with Fe3O4 magnetic aggregates of 0.7 ~ 0.8μm were used as seeds and styrene and divinylbenzene Body was added to the magnetic aggregates fine emulsion, the Fe3O4 magnetic aggregates were swollen after the polymerization was studied in the polymerization process, the swelling time on the morphology of composite microspheres and magnetic material content, to obtain a series of microspheres morphology by The prepared magnetic polymer microspheres were characterized by transmission electron microscopy (TEM), thermogravimetric analysis (TGA), infrared analysis (FTIR) and vibrating sample magnetometer (VSM) .The results showed that the obtained magnetic The size of polymer microspheres is 0.7-1μm, the size distribution is more uniform, with superparamagnetism, the content of magnetic substance is 29wt% ~ 57wt% .And then by the copolymerization of acrylic acid and styrene on the microsphere surface carboxyl functionalization, the surface The density of carboxyl group is 0.92mmol / g microspheres, and then the prepared microspheres conjugate with biomolecules (hCG as a model to be seized molecules), in the chemiluminescence immunoassay for a preliminary application, obtained Better application results.