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报道了基于纳米金-Nafion修饰金电极检测人端粒DNA的电化学阻抗传感器。将纳米金与Nafion混合超声得到纳米金-Nafion纳米材料,将此纳米材料滴涂于金电极表面获得纳米金-Nafion修饰电极。再将探针人端粒ss DNA滴涂在修饰电极上制备电化学阻抗传感器。利用扫描显微镜对纳米材料的形貌进行了表征。利用循环伏安法和电化学阻抗法对传感器进行了表征及目标人端粒DNA的定量测定。在最优化实验条件下,电化学阻抗传感器响应信号(ΔRet)与目标人端粒DNA浓度的对数(lgc)在0.001~1.0 nmol/L范围内呈良好线性关系。检出限为3.0 pmol/L。对0.5 nmol/L的目标人端粒DNA 7次平行测定,相对标准偏差RSD为3.5%。
An electrochemical impedance sensor based on nano-gold-Nafion modified gold electrode for detecting human telomeric DNA was reported. Nano gold and Nafion will be mixed ultrasound nano-gold Nafion nanomaterials, the nano-material was applied to the surface of the gold electrode to obtain gold-Nafion modified electrode. The probe human telomere ss DNA was then applied to the modified electrode to prepare an electrochemical impedance sensor. The morphology of nanomaterials was characterized by scanning microscope. The sensor was characterized by cyclic voltammetry and electrochemical impedance spectroscopy and the quantitative determination of target human telomere DNA. Under optimized experimental conditions, the linear relationship between the electrochemical impedance sensor response signal (ΔRet) and the logarithm of target human DNA concentration (lgc) was in the range of 0.001-1.0 nmol / L. The detection limit was 3.0 pmol / L. The relative standard deviation (RSD) was 3.5% for seven parallel assays of 0.5 nmol / L target human telomeric DNA.