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目的：观察习惯性流产患者接受主动免疫治疗后免疫反应性的变化。方法：采用改良的体外混合淋巴细胞培养（ＭＬＣ） 模拟临床主动免疫治疗，刺激后的患者淋巴细胞的反应能力用ＣｏｎＡ 检测。结果：经患者丈夫单核细胞刺激后的患者淋巴细胞对ＣｏｎＡ 的反应能力显著下降，与未经刺激的细胞相比，Ｐ＜ ０００１ 。同时，患者夫妇的ＭＬＣ上清对无关个体间的ＭＬＣ 具有抑制作用，抑制率为３６７ ％ 。结论：表明同种异体细胞的刺激能诱导出免疫抑制，这一结果与临床习惯性流产患者主动免疫治疗的结果相一致。
Objective: To observe the changes of immunoreactivity in active abortion patients after habitual abortion. Methods: Modified active in vitro mixed lymphocyte culture (MLC) was used to simulate clinical active immunotherapy. The responsiveness of lymphocytes after stimulation was detected by ConA. Results: The responsiveness of lymphocytes to Con A in patients with monocytes stimulated by their husbands was significantly lower than that of non-stimulated cells (P <0001). At the same time, the MLC supernatant of patients and couples had an inhibitory effect on MLC of unrelated individuals with the inhibition rate of 36.7%. CONCLUSIONS: This indicates that allogeneic cell stimulation induced immunosuppression, a finding consistent with the results of active immunotherapy in patients with habitual abortion.