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用48对SSR引物和50个RFLP探针对来自6个生态型的57份水稻材料之间的遗传差异进行了比较分析,从单个位点的等位基因数和平均基因多样性看,SSR的多态性要高于RFLP。从遗传距离看,在总计 1596对材料的遗传距离中,只有 144对(占9%)用 RFLP标记估计的遗传距离(简称GDRFLP,下同)高于用SSR标记估计的遗传距离(简称GDSSR,下同),平均高出的幅度为0.0371,而有 1452对(占 91%)材料间的 GDRFLP,高于 GDRFLP,平均高出幅度为0.2492。无论是籼、粳亚种内还是籼粳亚种间,SSR标记比RFLP估计的遗传距离高.如用SSR标记估计的籼稻亚种内材料之间平均遗传距离为0.5483,而RFLP标记估计的只有0.2776;粳稻亚种内SSR平均遗传距离为0.5777,RFLP的只有0.2405;用SSR和RFLP估计的亚种间的平均遗传距离分别为0.8074和0.731。成对材料的SSR遗传距离与RFLP遗传距离之间的相关分析表明,对于典型的籼粳品种,这两种标记估计的遗传距离相关系数较高,而中间型材料或偏籼偏粳材料的相关程度较低。通过分析4个两系不育系与48个恢复系之间的遗传距离与F
48 pairs of SSR primers and 50 RFLP probes were used to compare the genetic differences among 57 rice materials from 6 ecotypes. According to the number of alleles and the average genetic diversity of single locus, SSR Polymorphism is higher than RFLP. From the genetic distances, only a total of 1596 genetic distances (9%) were estimated to be higher than the genetic distance estimated by SSR markers (referred to as GDSSR, The same below), the average amplitude was 0.0371, and 1452 pairs (91%) of the GDRFLP between materials, higher than the GDRFLP, an average height of 0.2492. The SSR markers were higher than those estimated by RFLP in both indica and japonica subspecies or between indica and japonica subspecies. For example, the average genetic distance of indica subsp. Japonica subspecies estimated by SSR markers was 0.5483, while that of RFLP markers was only 0.2776. The average SSR of japonica subspecies was 0.5777 and that of RFLP was only 0.2405 The average genetic distance between subspecies estimated by SSR and RFLP was 0.8074 and 0.731, respectively. The correlation analysis between SSR genetic distance and RFLP genetic distance of paired materials showed that the correlation coefficient of genetic distance between the two markers was high for typical indica and japonica varieties, To a lesser extent. By analyzing the genetic distance between four lines and 48 restorers,