论文部分内容阅读
目的:探讨两种中药方剂对MRL/lpr狼疮小鼠的治疗作用及其作用机制,为中药及中西医结合治疗红斑狼疮提供实验和理论基础。方法:34只MRL/lpr狼疮小鼠随机分为6组。A组:中药方1组(清热凉血、祛风除湿方);B组:中药方2组(养阴清热活血、滋补肝肾方);C组:泼尼松组;D组:中药方1+泼尼松组;E组:中药方2+泼尼松组;F组:空白对照组。ELISA法测小鼠血浆中抗dsDNA抗体的含量;对小鼠肾脏做普通组织病理及免疫病理检测,观察各组小鼠肾脏的变化;各组每10天用尿蛋白试纸比色(四溴酚兰指示剂法)测定新鲜尿液的蛋白质含量。结果:各药物组治疗后的抗dsDNA抗体水平均低于空白对照组(P<0.05)。中药方1+泼尼松组和中药方2+泼尼松组的抗体水平均显著低于中药方1组、中药方2组(P<0.01),低于泼尼松组(P<0.05)。各给药组尿蛋白的含量均明显低于空白对照组。肾脏组织病理检查发现各给药组均可减轻小鼠肾脏损害,其中以中药+泼尼松组最为明显。结论:中药方剂与糖皮质激素均对MRL/lpr狼疮小鼠有治疗作用,中西医综合治疗疗效优于单纯使用中药或糖皮质激素,能显著降低抗dsDNA抗体水平和减轻肾脏损害。
Objective: To explore the therapeutic effect and mechanism of two traditional Chinese medicine prescriptions on MRL / lpr lupus mice and to provide experimental and theoretical basis for the treatment of lupus erythematosus with traditional Chinese medicine and traditional Chinese and western medicine. Methods: Thirty-four MRL / lpr lupus mice were randomly divided into 6 groups. Group A: Chinese medicine group 1 (clearing heat, expelling wind and dehumidification side); Group B: Chinese medicine group 2 (Yin Yin heat heat, nourishing liver and kidney); C group: prednisone group D: 1+ prednisone group; E group: traditional Chinese medicine 2 + prednisone group; F group: blank control group. ELISA method was used to measure the content of anti-dsDNA antibody in the plasma of mice. The histopathology and immunopathological examination were performed on the kidneys of mice to observe the changes of the kidneys in each group. Urine protein colorimetric assay (tetrabromophenol Blue indicator method) Determination of fresh urine protein content. Results: The levels of anti-dsDNA antibody in each drug group were lower than those in the blank control group (P <0.05). The antibody levels of Prescription 1 + prednisone and traditional Chinese medicine 2 + prednisone were significantly lower than those of traditional Chinese medicine Prescription 1 and Chinese Prescription 2 (P <0.01) and lower than that of prednisone (P <0.05) . The content of urinary protein in each administration group was significantly lower than that in the blank control group. Kidney histopathology examination found that each administration group can reduce kidney damage in mice, of which traditional Chinese medicine + prednisone group was the most obvious. Conclusion: The prescriptions of Chinese medicine and glucocorticoids both have a therapeutic effect on MRL / lpr lupus mice, and the curative effect of integrated traditional Chinese and western medicine is better than that of traditional Chinese medicine or glucocorticoid alone. It can significantly reduce the anti-dsDNA antibody level and reduce the renal damage.