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以贝母素乙为模板分子,环氧树脂为功能单体、聚乙二醇1000为致孔剂、二乙烯三胺为交联剂,通过逐步聚合法合成了贝母素乙的分子印迹聚合物。在流动注射化学发光仪的通路中,放置贝母素乙MIP,创建新型的流通式贝母素乙的分子印迹化学传感器,用于检测贝母属类样品中的贝母素乙。对贝母素乙的吸附量和结合位点用Scatchard方法进行了详细分析。方法的线性范围为4.0×10-5~2.0×10-3mol/L,检出限为3.0×10-6mol/L。对3.0×10-4mol/L贝母素乙溶液的浓度平行测定了11次,RSD为2.3%。此方法可成功应用于川贝母、浙贝母和暗紫贝母中贝母素乙含量的测定,加标回收率为96.5%~103.6%,分析结果令人满意。
The molecularly imprinted polymerization of peiminine B was synthesized by a stepwise polymerization using peiminine as a template molecule, epoxy resin as a functional monomer, polyethylene glycol 1000 as a porogen and diethylenetriamine as a cross-linking agent Things. In flow-through chemiluminescenters, place the Fritillaria-MIP and create a new flow-through molecular marker chemistry for the detection of Fritillaria B in Fritillaria samples. Adsorption amount and binding sites on peiminine B were analyzed in detail using the Scatchard method. The linear range of the method was 4.0 × 10-5 ~ 2.0 × 10-3mol / L with the detection limit of 3.0 × 10-6mol / L. The concentration of 3.0 × 10-4 mol / L parental B solution was determined in parallel 11 times with a RSD of 2.3%. The method was successfully applied to the determination of peimine B in Fritillaria cirrhosa, Fritillaria ussuriensis Maxim. And Fritillaria ussuriensis Maxim with the recoveries of 96.5% -103.6%. The analytical results were satisfactory.