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目的对2008-2013年四川省登革热病例的实验室检测结果进行总结分析。方法收集2008-2013年由华西医院、四川省人民医院等收治的34例登革热住院患者的血清标本,用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测登革病毒的血清Ig M抗体,实时荧光PCR检测病毒核酸,并进行总结分析。结果 34例病例共收集41份血清标本,患者男女比例为3.86∶1,平均年龄37.24岁,病例全年均有。ELISA检测41份标本中31份结果为阳性(阳性率为75.61%);实时荧光PCR检测的21份标本中,8份结果为阳性(阳性率为38.10%)。最后实验室诊断病例为26例。结论不同病程阶段的病例选择不同的方法进行检测,可有效提高诊断登革病毒感染的敏感性。
Objective To summarize the laboratory test results of dengue fever cases in Sichuan Province from 2008 to 2013. Methods Serum samples of 34 dengue inpatients admitted from West China Hospital and Sichuan Provincial People’s Hospital from 2008 to 2013 were collected. Serum Ig M antibody of dengue virus was detected by enzyme-linked immunosorbent assay (ELISA) , Real-time fluorescent PCR detection of viral nucleic acid, and carry out summary analysis. Results A total of 41 serum samples were collected from 34 patients. The ratio of male to female was 3.86:1, with an average age of 37.24 years. The cases were all year round. 31 of 41 samples were positive by ELISA (positive rate was 75.61%). Of 21 samples detected by real-time fluorescence PCR, 8 were positive (the positive rate was 38.10%). The final laboratory diagnosis of cases of 26 cases. Conclusions Different cases of different stages of disease can be tested by different methods, which can effectively improve the sensitivity of diagnosis of dengue virus infection.