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作者从黄花夹竹桃属植物Thevetia ahouia A.DC的乙醚提取物中,分得一种新的细胞毒强心甙,命名为3′-O-methylevomonoside(Ⅰ)。Ⅰ经药理实验证明在2.7×10~(-3)μg/ml剂量时,对KB细胞具有很强的活性,ED_(50)≤20μg/ml。5月间从墨西哥采集Thevetia ahouia A.DC地上部分2500g,于索氏提取器中用乙醚提取,经真空干燥得乙醚提取物150g,加正己烷磁力搅拌4小时,放置过夜,过滤,残余物用正己烷洗脱,滤液真空于燥后,用二氯甲烷搅拌6小时,过滤,真空干燥,残余物经
The authors obtained a new cytotoxic cardiac glycoside named as 3’-O-methylevomonoside (Ⅰ) from the ether extract of Thevetia ahouia A.DC. Ⅰ Pharmacological experiments showed that the cells were highly active against KB cells at a dose of 2.7 × 10 -3 μg / ml, with ED 50 ≤20μg / ml. In May, collected from Mexico, Thevetia ahouia A.DC above ground 2500g, extracted with ether in a Soxhlet extractor, dried under vacuum to give ether extract 150g, plus n-hexane magnetic stirring for 4 hours, placed overnight, filtered and the residue Hexane, the filtrate was dried in vacuo, stirred with dichloromethane for 6 hours, filtered and dried under vacuum. The residue was purified by chromatography