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目的观察人恶性黑色素瘤组织内血管内皮生长因子C(VEGF-C)及其受体3(VEGFR-3)的表达,探讨VEGF-C和VEGFR-3在恶性黑色素瘤淋巴管生成及淋巴道转移中的作用。方法取人恶性黑色素瘤组织48例(石蜡标本30例,术后新鲜组织18例),应用免疫组织化学和RT-PCR技术,观察VEGF-C和VEGFR-3蛋白及mRNA在恶性黑色素瘤组织内的表达情况。以淋巴管内皮透明质酸受体(LYVE-1)标记淋巴管,计数恶性黑色素瘤组织淋巴管数密度。结果VEGF-C和VEGFR-3蛋白主要表达于恶性黑色素瘤细胞胞浆内,在肿瘤周围的血管和淋巴管内皮上也可见VEGFR-3蛋白表达,VEGF-C和VEGFR-3蛋白在淋巴结转移组恶性黑色素瘤组织内的表达水平明显高于无淋巴结转移组(P<0.05)。在18例新鲜恶性黑色素瘤中,淋巴结转移组VEGF-C和VEGFR-3mRNA的表达明显高于无淋巴结转移组(P<0.01)。LYVE-1表达于肿瘤间质内的淋巴管内皮细胞,淋巴结转移组恶性黑色素瘤组织中的淋巴管数密度(LMVD)为9.845±2.454,无淋巴结转移组恶性黑色素瘤组织中的淋巴管数密度为6.534±2.193,淋巴结转移组恶性黑色素瘤组织内的淋巴管数密度明显高于无淋巴结转移组(P<0.01)。结论恶性黑色素瘤组织内VEGF-C表达明显增高,并通过上调其受体VEGFR-3的表达促进恶性黑色素瘤组织内淋巴管的生成,从而促进恶性黑色素瘤的淋巴道转移。
Objective To observe the expression of vascular endothelial growth factor C (VEGF-C) and its receptor 3 (VEGFR-3) in human malignant melanoma and to explore the role of VEGF-C and VEGFR-3 in lymphangiogenesis and lymphatic metastasis of malignant melanoma In the role. Methods Forty-eight patients with malignant melanoma (30 paraffin-embedded specimens and 18 fresh ones) were obtained. The expressions of VEGF-C and VEGFR-3 protein and mRNA in malignant melanoma tissues were detected by immunohistochemistry and RT-PCR The expression of the situation. Lymphatic vessels were labeled with lymphatic endothelial hyaluronic acid receptor (LYVE-1), and the number density of lymphatic vessels in malignant melanoma tissues was counted. Results VEGF-C and VEGFR-3 protein were mainly expressed in the cytoplasm of malignant melanoma cells. The expression of VEGFR-3 protein was also observed on the vascular and lymphatic endothelium around the tumor. The expressions of VEGF-C and VEGFR-3 protein in the lymph node metastasis group The expression level of malignant melanoma tissue was significantly higher than that without lymph node metastasis (P <0.05). In 18 cases of fresh malignant melanoma, the expression of VEGF-C and VEGFR-3 mRNA in lymph node metastasis group was significantly higher than that in non-lymph node metastasis group (P <0.01). LYVE-1 was expressed in the lymphatic endothelial cells in the interstitial of the tumor. The number of lymphatic vessels (LMVD) in lymphatic metastasis malignant melanoma tissue was 9.845 ± 2.454, and the number of lymphatic vessels in lymphatic metastasis-free malignant melanoma tissue Was 6.534 ± 2.193. The number density of lymphatic vessels in malignant melanoma tissues of lymph node metastasis was significantly higher than that of non-lymph node metastasis (P <0.01). Conclusion The expression of VEGF-C in malignant melanoma tissues is significantly increased. The up-regulation of VEGFR-3 expression in malignant melanoma can promote lymphangiogenesis in malignant melanoma tissues and promote the lymphatic metastasis of malignant melanoma.