目的 :探讨分叉双歧杆菌 (B bifidum)的全肽聚糖对巨噬细胞功能的调节作用。方法 :首先用全肽聚糖注射于裸鼠腹腔 ,以ELISA法测定裸鼠腹腔巨噬细胞产生的IL - 6和IL - 12含量 ,同时用Griess试剂检测一氧化氮 (NO)的水平。结果 :全肽聚糖注射组裸鼠腹腔巨噬细胞产生的IL - 6、IL - 12及NO含量分别为732 5 4± 190 30 (pg/mL)、816 37± 96 40 (pg/mL)和 48 90± 6 5 1(μmol/L) ;对照组分别为 30 3 78± 171 75 (pg/mL)、5 10 2 7± 12 3 46 (pg/mL)以及 30 6 7± 12 83(μmol/L) ,三者比较均具有显著差异 (P <0 0 1)。 结论 :分叉双歧杆菌的全肽聚糖能激活巨噬细胞 ,使之分泌多量的IL - 6、IL - 12及NO ,这些细胞毒性效应分子介导了全肽聚糖的多种重要生理功能。 Objective: To investigate the regulation of macrophage function by peptidoglycan of bifidum bifidum. Methods: The peritoneal cavity of nude mice was injected with peptidoglycan at first. The levels of IL - 6 and IL - 12 in peritoneal macrophages of nude mice were determined by ELISA. The level of nitric oxide (NO) was detected by Griess reagent. Results: The levels of IL - 6, IL - 12 and NO in peritoneal macrophages injected with whole peptidoglycan in nude mice were 732 54 ± 190 30 (pg / mL) and 816 37 ± 96 40 (pg / mL) And 48 90 ± 6 5 1 (μmol / L) respectively; the control group was 30 3 78 ± 171 75 (pg / mL), 5 10 2 7 ± 12 3 46 (pg / mL) and 30 6 7 ± 12 83 μmol / L). There were significant differences among the three groups (P <0.01). CONCLUSION: The whole peptidoglycan of Bifidobacterium bifidum activates macrophages to secrete a large amount of IL - 6, IL - 12 and NO. These cytotoxic effect molecules mediate many important physiological activities of peptidoglycan Features.