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目的研究肠道菌群变化与结、直肠肿瘤之间的关系。方法病理检查证实的结、直肠肿瘤患者35例为研究组,同期结肠镜检查排除结、直肠肿瘤的患者35例为对照组。收集两组患者的粪便标本,采用扩增核糖体DNA限制性分析(ARDRA)技术对样本内细菌进行检测,比较两组菌种的变化及规律。结果两组70例样本共检出389 010条高质量序列,研究组和对照组Chao1指数分别为1.01×10~3和0.85×10~3,Shannon指数分别为0.11×10~2和0.14×10~2,两组菌群多样性和均匀性比较差异无统计学意义(P>0.05)。而在菌种/属水平,有15种细菌丰度值比较差异有统计学意义(P<0.05)。结论结直肠肿瘤患者肠道某些菌种与正常人存在差异,研究其变化规律有可能实现对结、直肠肿瘤进行早期的预防、早期诊断,并为微生态制剂作为结、直肠肿瘤的治疗方式提供依据。
Objective To study the relationship between intestinal flora changes and colorectal tumors. Methods Twenty-five patients with colorectal neoplasm confirmed by histopathological examination were included in the study group, and 35 patients with colorectal neoplasms and colorectal neoplasms during the same period were selected as the control group. The stool samples of two groups were collected and the bacteria in the samples were detected by the amplified ribosomal DNA restriction analysis (ARDRA). The changes and regularities of the two strains were compared. Results A total of 389 010 high quality sequences were detected in 70 samples from two groups. The Chao1 index of the study group and the control group were 1.01 × 10-3 and 0.85 × 10-3, respectively, and Shannon’s index was 0.11 × 10-2 and 0.14 × 10 ~ 2, there was no significant difference in the diversity and uniformity between the two groups (P> 0.05). In the species / genus, there are 15 kinds of bacteria abundance value difference was statistically significant (P <0.05). Conclusions There are some differences in intestinal tract between colorectal cancer patients and normal individuals. It is possible to study the variation regularity of colorectal cancer in early stage of prevention and early diagnosis of colorectal and rectal cancer and to treat the probiotics as the treatment of colorectal and rectal tumors Provide evidence.