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目的:探讨NaHS对子宫颈癌细胞增殖的影响及其机制。方法:首先将子宫颈癌HeLa细胞分成对照组和不同剂量的NaHS处理组,并采用MTT法检测细胞存活率和流式细胞仪检测细胞凋亡率。使用不同浓度的格列本脲预先处理HeLa细胞30 min,再用400μmol/L的NaHS处理24 h,探讨NaHS抑制子宫颈癌细胞增殖的初步机制。结果:不同剂量的NaHS处理组能明显抑制HeLa细胞增殖而促进HeLa细胞凋亡;格列本脲预先处理可以部分阻断这种作用。结论:NaHS抑制子宫颈癌细胞增殖可能与ATP敏感性钾通道有关。
Objective: To investigate the effect of NaHS on the proliferation of cervical cancer cells and its mechanism. Methods: Cervical cancer HeLa cells were divided into control group and different doses of NaHS treatment group, and the cell viability was detected by MTT assay and the apoptosis rate was detected by flow cytometry. HeLa cells were pretreated with different concentrations of glyburide for 30 min and then treated with 400 μmol / L NaHS for 24 h to investigate the primary mechanism of NaHS inhibiting the proliferation of cervical cancer cells. Results: Different doses of NaHS treatment group could significantly inhibit the proliferation of HeLa cells and promote the apoptosis of HeLa cells. Pretreatment with glyburide could partially block this effect. Conclusion: NaHS inhibits the proliferation of cervical cancer cells may be related to ATP-sensitive potassium channels.