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目的 观察生长抑素（ＳＯＭ）是否和如何参与对人脐静脉（ＨＵＶ）张力的调节及是否存在ＨＵＶ 局部来源的ＳＯＭ。 方法 应用机械电换能器记录ＳＯＭ 对ＨＵＶ张力的影响及应用原位杂交（ＩＳＨ）技术观察体外培养人脐静脉内皮细胞（ＨＵＶＥＣ）是否表达ＳＯＭ 的ｍ ＲＮＡ。 结果 当存在ＨＵＶＥＣ时，ＳＯＭ 使ＨＵＶ明显舒张；不存在ＨＵＶＥＣ时，ＳＯＭ 并不使ＨＵＶ明显舒张。原位杂交显示体外培养ＨＵＶＥＣ出现ＳＯＭ ｍ ＲＮＡ的阳性信号，其阳性百分率受血管活性多肽（ＶＰｓ）的调节。 结论 ＨＵＶＥＣ具有合成ＳＯＭ 的能力并成为ＨＵＶ 局部ＳＯＭ 的一个重要来源，ＳＯＭ 参与了对ＨＵＶ 张力的局部调节作用（ＨＵＶＥＣ依赖性舒张ＨＵＶ），并可能存在自身反馈机制。
Objective To investigate whether and how somatostatin (SOM) participates in the regulation of human umbilical vein (HUV) tension and the presence of SOM with local source of HUV. Methods The mechanical impedance transducer (SOM) was used to record the effect of SOM on HUV tension and the in situ hybridization (ISH) technique was used to observe the mRNA expression of SOM in human umbilical vein endothelial cells (HUVEC). Results SOM caused a significant relaxation of HUV in the presence of HUVEC; SOM did not significantly relax HUV in the absence of HUVEC. In situ hybridization showed positive signal of SOM m RNA in HUVEC cultured in vitro, and the positive percentage was regulated by vasoactive peptides (VPs). Conclusion HUVEC has the ability to synthesize SOM and become an important source of local SOM in HUV. SOM participates in the local regulation of HUV tension (HUVEC-dependent relaxation of HUV) and may have its own feedback mechanism.