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【目的】鉴定荔浦芋疫病病原及制备原生质体,为荔浦芋病原检测、致病机理研究及健康种苗生产提供科学依据。【方法】对采集自广西荔浦县芋头种植区的芋疫病标样进行分离,通过形态特征和r DNA-ITS分子生物学相结合的方法对其病原进行鉴定,同时对获得的病原菌进行原生质体制备。【结果】通过对分离的芋疫病病原进行致病性测定、形态特征观察,将引起荔浦芋疫病的病原初步鉴定为芋疫霉菌(Phytophthora colocasiae);r DNA-ITS序列分析结果表明,菌株DNA序列与Gen Bank已发表的P.colocasiae不同分离物序列同源性达99%,进一步确定所测菌株为芋疫霉菌。制备获得的芋疫霉菌原生质体呈透明圆形或近圆形,大小不一。【结论】引起荔浦芋疫病的病原为芋疫霉菌,制备的芋疫霉菌原生质体可用于芋疫霉病致病机理研究。
【Objective】 The objective of this study was to identify the pathogen of Phytophthora blight and to prepare protoplasts for the detection of pathogenic pathogens and the production of healthy seedlings. 【Method】 Taro samples collected from Taro planting area in Lipu County of Guangxi Province were isolated, and the pathogen was identified by morphological characteristics and molecular biology of r DNA-ITS. Meanwhile, the protoplasts preparation. 【Result】 The pathogenicity and morphological characteristics of the isolated taro pathogen were identified and the pathogen causing the disease was identified as Phytophthora colocasiae. The results of r DNA-ITS sequence analysis showed that the DNA The sequence shared 99% homology with the published isolates of P.colocasiae in Gen Bank, which further confirmed that the tested strain was P. taro. Protoplasts of P. taro obtained were either transparent or nearly round in shape and size. 【Conclusion】 The pathogen of Phytophthora infestans is Phytophthora infestans. The protoplast of Phytophthora infestans can be used to study the pathogenesis of Phytophthora infestans.