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为探讨白塞病是否存在特异性循环自身抗体,我们应用免疫印迹结合免疫沉淀技术对39例该病患者血清进行了初步筛查。结果发现,分别有8份(20.5％)、9份(23.1％)血清识别相对分子质量约为100 000、50 000的细胞内性质不明成分,其中4份标本(10.3％)与上述两种蛋白反应;在免疫沉淀分析中,15份血清(38.5％)可主要沉淀细胞中相对分子质量约为200 000、160 000、120 000、100 000的某些蛋白,部分免疫印迹阳性的血清其免疫沉淀分析也呈阳性结果。研究提示白塞病血清中可能存在作用于细胞内未知抗原的IgG型自身抗体,值得深入探索,以澄清靶抗原的性质,明确相应自身抗体对白塞病的特异性、血清学诊断价值及其可能的免疫致病机制。
In order to investigate whether there is a specific circulating autoantibody in Behcet’s disease, we conducted a preliminary screening of the serum of 39 patients with this disease by Western blotting and immunoprecipitation. As a result, 8 (20.5%) and 9 (23.1%) sera were identified as unknown intracellular components with a relative molecular mass of about 100,000 and 50,000, respectively. Four of the samples (10.3%) and the two proteins In the immunoprecipitation assay, 15 serums (38.5%) mainly precipitated certain proteins with relative molecular mass of about 200 000, 160 000, 120 000 and 100 000 in cells, and some of the immunoprecipitated sera immunoprecipitated Analysis also positive results. The research suggests that there may be IgG-type autoantibodies that act on unknown intracellular antigens in Behcet’s disease serum, and it is worth further exploration to clarify the nature of the target antigens, clarify the specificity of the corresponding autoantibodies to Behcet’s disease, serological diagnostic value and its possible Immune pathogenesis.