自1941年以来,荧光抗体技术应用于医学和生物学的研究,至今已有三十多年。该项技术近年来发展很快, 目前国内部分医院已把它作为检查自身抗体的常规方法。能较快地测出少量自身抗体,具有抗原抗体反应的特异性,染色的快速性和在细胞水平定位的灵敏性等优点。检查原理也较简单,即将合适的组织抗原切片或涂片用病人的血清处理后,再用荧光标记抗人丙球蛋白液做间接荧 Fluorescent antibody technology has been used in medical and biological research since 1941 for more than three decades. The technology has developed rapidly in recent years, at present, some hospitals in China have used it as a routine method to check their antibodies. Can quickly detect a small amount of autoantibodies, with the specificity of the antigen-antibody reaction, the rapidity of staining and the sensitivity of the level of cellular positioning and so on. Check the principle is relatively simple, the appropriate tissue antigen slices or smears with the patient’s serum after treatment, and then fluorescently labeled anti-human globulin indirect indirect fluorescence