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目的:研究吗啡对雌激素诱导的体外培养人的正常乳腺细胞的雌激素受体(ER)mRNA表达的影响。方法:购买人正常乳腺纤维细胞株进行培养,给予17-β雌二醇(E2),吗啡(Mor)及纳洛酮干预。通过RT-PCR的方法半定量分析吗啡对正常乳腺细胞ERmRNA表达的影响。结果:ER与内参照B-actin分别在249bp和270bp处出现特征性条带。雌激素组、吗啡组与对照组比较,差异有显著性(P<0.05),E2+Mor组与对照组比较,差异无显著性(P>0.05)。吗啡作用24h呈剂量依赖性抑制乳腺细胞ERmRNA的表达,3组吗啡的终浓度分别为1×10-6mol/L、1×10-5mol/L、1×10-4mol/L,差异有显著性(P<0.05)。结论:从mRNA水平证明了外源性阿片类药物吗啡可直接影响雌激素对乳腺的促增殖作用,同时还可剂量依赖性下调雌激素受体mRNA的表达。
Objective: To study the effect of morphine on estrogen-induced estrogen receptor (ER) mRNA expression in human normal breast cells. METHODS: Normal human breast fibroblasts were purchased and cultured for the intervention of 17-β estradiol (E2), morphine and naloxone. The effects of morphine on the expression of ER mRNA in normal breast cells were semi-quantitatively analyzed by RT-PCR. Results: The characteristic bands of ER and internal reference B-actin appeared at 249bp and 270bp respectively. There was significant difference between estrogen group and morphine group and control group (P <0.05). There was no significant difference between E2 + Mor group and control group (P> 0.05). Morphine could inhibit the expression of ERmRNA in breast cancer cells in a dose-dependent manner for 24h. The final concentrations of morphine in the three groups were 1 × 10-6mol / L, 1 × 10-5mol / L and 1 × 10-4mol / L, respectively, with significant difference (P <0.05). CONCLUSION: Morphine, an exogenous opioid, has been shown to directly affect the pro-proliferative effects of estrogen on mammary glands and down-regulate estrogen receptor mRNA expression in a dose-dependent manner from mRNA levels.