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目的建立HPLC同时测定复方鱼腥草片中绿原酸、连翘酯苷A、槲皮苷和黄芩苷含量的方法。方法采用CAPCELL PAK MG(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈(A)-0.1%磷酸溶液(B),梯度洗脱(0~12 min,9%A;12~15 min,9%→15%A;15~32 min,15%A;32~48 min,15%→29%A;48~60 min,29%A),流速1.0 m L·min~(-1),柱温35℃,检测波长:330 nm。结果绿原酸、槲皮苷浓度在2~100μg·m L-1内,连翘酯苷A浓度在4~200μg·mL~(-1)内,黄芩苷浓度在12~600μg·m L-1内,与峰面积均呈良好的线性关系(r=0.999 9);平均回收率(n=6)分别为98.0%,99.8%,99.1%和98.3%,RSD均<2.0%。结论建立的HPLC可实现同时测定绿原酸、连翘酯苷A、槲皮苷和黄芩苷4种有效成分,方法快速、简便、结果准确,可为复方鱼腥草片提供更全面、可靠的质量控制方法。
Objective To establish a method for the simultaneous determination of chlorogenic acid, forsythoside A, quercitrin and baicalin in compound Houttuynia cordata Thunb. Methods The mobile phase consisted of acetonitrile (A) - 0.1% phosphoric acid solution (B) with gradient elution (0-12 min, 9% A, 12-15 min) on a CAPCELL PAK MG (4.6 mm × 250 mm, , 9% → 15% A; 15-32 min, 15% A; 32-48 min, 15% → 29% A; 48-60 min, 29% , Column temperature 35 ℃, detection wavelength: 330 nm. Results The concentration of chlorogenic acid and quercitrin was in the range of 2 ~ 100μg · m L -1, the concentration of forsythiaside A was in the range of 4 ~ 200μg · mL -1, the concentration of baicalin was in the range of 12 ~ 600μg · m L- (R = 0.999 9). The average recoveries (n = 6) were 98.0%, 99.8%, 99.1% and 98.3%, respectively, with a RSD of 2.0%. Conclusion The established HPLC can be used for the simultaneous determination of 4 active ingredients of chlorogenic acid, forsythiaside A, quercitrin and baicalin. The method is rapid, simple and accurate. It can provide more comprehensive and reliable Quality control methods.