The chromatographic behavior of twenty three amino acids on the silica gel thin layers using sodium dodecylsulfate(SDS)/ n butyl alcohol/ n hexane/water microemulsion as a developer have been studied. The results showed that the W/O microemulsion and the bicontinuous microemulsion composed of the mentioned components appeared suitable developers for TLC analysis of amino acids. The chromatographic behavior of twenty three amino acids on the silica gel thin layers using sodium dodecylsulfate (SDS) / n butyl alcohol / n hexane / water microemulsion as a developer have been studied. The results showed that the W / O microemulsion and the bicontinuous microemulsion composed of the mentioned components appeared suitable developers for TLC analysis of amino acids.