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采用气相色谱-串联质谱(GC-MS/MS)技术建立了同时检测7种类型蔬菜中氟吡菌酰胺及其代谢物残留的分析方法。样品采用乙腈提取,无水硫酸镁和氯化钠盐析后,经分散固相萃取(PSA)净化,GC-MS/MS检测。结果表明:在0.01、0.1和1 mg/kg 3个添加水平下,氟吡菌酰胺及其代谢物2-(三氟甲基)苯甲酰胺(BZM)的平均回收率在80%~108%之间,相对标准偏差(RSD)在1.5%~8.4%之间。氟吡菌酰胺及其代谢物在7种蔬菜中的检出限(LOD)(S/N=3)分别为2.17~3.13μg/kg和1.22~2.04μg/kg,两者的定量限(LOQ)均为0.01 mg/kg。该方法操作简便、稳定和快速,可以满足大部分蔬菜中氟吡菌酰胺及其代谢物的定性与定量分析要求。
Gas chromatography-tandem mass spectrometry (GC-MS / MS) was used to establish an analytical method for the simultaneous determination of fluopyram and its metabolites in seven types of vegetables. The sample was extracted with acetonitrile, salted with anhydrous magnesium sulfate and sodium chloride, then purified by dispersive solid-phase extraction (PSA) and detected by GC-MS / MS. The results showed that the average recoveries of fluopylucan and its metabolites 2- (trifluoromethyl) benzamide (BZM) at the concentrations of 0.01, 0.1 and 1 mg / kg were 80% -108% Between the relative standard deviation (RSD) of 1.5% to 8.4%. The LOD (S / N = 3) of fluopyram and its metabolites were 2.17 ~ 3.13μg / kg and 1.22 ~ 2.04μg / kg respectively in seven kinds of vegetables. The limits of quantification ) Were 0.01 mg / kg. The method is simple, stable and fast to meet the requirements of qualitative and quantitative analysis of fluopyram and its metabolites in most vegetables.