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采用氯胺-T法对白蛋白融合干扰素α2b进行125I标记,PD-10柱纯化,三氯醋酸沉淀法测定放化纯;体外以WISH/VSV系统比较白蛋白融合干扰素α2b和125I-白蛋白融合干扰素α2b的抗病毒活性;SD大鼠皮下注射125I-白蛋白融合干扰素α2b,分别于给药后0.5、2、6、24、48、90、180、300h放血处死,取血和相关脏器,称重并测定放射性,计算每克脏器放射性占注入量的百分率(%ID?g-1)。结果显示,氯胺-T法制备的125I-白蛋白融合干扰素α2b标记率为82.72%,放化纯95.53%,放射性比活度为0.26MBq/μg;抗病毒活性比较结果表明标记前后的生物学活性几乎无变化;皮下注射大鼠体内分布研究表明,125I-白蛋白融合干扰素α2b在血中于6h达高峰,消除缓慢,未见特异性组织或器官的浓聚。
The albumin fusion interferon α2b was labeled with 125I by chloramine-T method, purified by PD-10 column and radiolabeled by trichloroacetic acid precipitation method. In vitro, albumin fusion interferon α2b and 125I-albumin were compared with WISH / VSV system Fusion of interferon α2b antiviral activity; SD rat subcutaneous injection of 125I-albumin fusion interferon α2b, respectively, after administration of 0.5,2,6,24,48,90,180,300h bloodletting, bleeding and related The organs were weighed and radioactivity was measured. The percentage of radioactivity per gram of organism (% ID? G-1) was calculated. The results showed that the labeling rate of fusion interferon α2b was 82.72%, the radiochemical purity was 95.53%, and the specific activity of radioactivity was 0.26MBq / μg. The results of antiviral activity showed that the biological The results showed that 125I-albumin fusion interferon α2b peaked in blood at 6h, and it disappeared slowly, and no specific tissue or organ concentration was observed.