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目的:为临床诊断乙型肝炎提供灵敏、准确、可靠的实验依据。方法:采用PCR杂交梳(反相膜杂交法),对71例乙型肝炎患者血清进行DNA验证性检测,并与一般聚合酶链反应(PCR琼脂糖凝胶电泳法)同步对比分析。结果:71例乙型肝炎患者血清中杂交梳方法检出HBV DNA阳性58例,阳性率81.7％;琼脂糖凝胶电泳质检出HBV DNA阳性50例,阳性率70％。结论:PCR杂交梳法较PCR法具有更高特异性和敏感性。
Objective: To provide a sensitive, accurate and reliable experimental basis for clinical diagnosis of hepatitis B. Methods: Serum samples from 71 patients with hepatitis B were tested by DNA hybridization with reverse hybridization (PCR), and analyzed by polymerase chain reaction (PCR agarose gel electrophoresis). Results: The positive rate of HBV DNA was detected in 58 out of 71 cases of hepatitis B patients by hybridization comb method, the positive rate was 81.7%. The positive rate of HBV DNA was 70% by agarose gel electrophoresis. Conclusion: PCR hybridization is more specific and sensitive than PCR.