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目的探讨CYP27B1(Cytochromo P450 CYP)基因启动子C-1260A位点多态性与宁夏汉族Graves病(GD)的相关性。方法采用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction restriction frag-ment length polymorphism,PCR-RFLP)技术,分析汉族GD 99例和正常对照83例CYP27B1基因启动子-1260C/A多态性。结果宁夏汉族CYP27B1基因-1260C/A检出GD组与对照组的基因型频率分别为37.37%、53.54%、9.09%和43.37%、40.96%、15.66%,两组差异无统计学意义(χ2=4.19,P>0.05);等位基因频率为64.16%、35.86%和63.86%、43.14%,两组差异无统计学意义(χ2=0.00,P>0.05)。结论 CYP27B1基因1260C/A的与宁夏地区汉族GD无相关性。
Objective To investigate the association between C-1260A polymorphism of CYP27B1 (Cytochromo P450 CYP) gene promoter and Graves’ disease (GD) in Han Ningxia. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to analyze the association between CYP27B1 gene promoter -1260C / A and GD99 in Han nationality and in 83 normal controls State. Results The genotype frequency of CYP27B1 gene -1260C / A in Ningxia Han nationality was 37.37%, 53.54%, 9.09% and 43.37%, 40.96% and 15.66%, respectively. There was no significant difference between the two groups (χ2 = 4.19, P> 0.05). The frequency of alleles was 64.16%, 35.86% and 63.86%, 43.14% respectively. There was no significant difference between the two groups (χ2 = 0.00, P> 0.05). Conclusion There is no correlation between CYP27B1 gene 1260C / A and GD in Ningxia.