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目的:建立高效液相色谱法测定兔骨组织中的克林霉素,并对其在使用止血带后,在骨组织的分布特征进行研究。方法:以乙腈-四氢呋喃-磷酸盐缓冲溶液(30∶1∶70,pH 5)为流动相,柱温40℃;流速0.8mL·min-1;以替硝唑为内标,以LunaC18柱(250mm×4.6mm,5μm)进行分离,检测波长为205nm。测定静脉注射克林霉素(20mg·kg-1)后30,60,90,120min(A、B、C、D)上止血带分别阻断血液循环30,60,90min实验组与未止血对照组的骨组织中药物含量。结果:克林霉素在10.58～264.5μg·mL-1范围内线性关系良好,日内RSD≤2.17%,日间RSD≤2.99%。实验组克林霉素含量与对照组比较,差异有极显著性(P<0.01);相同止血时机,不同止血时间30,60,90min组之间比较,无显著性差异(P>0.05);不同止血时机组,A组与B、C、D组比较差异有极显著性(P<0.01),B、C、D组骨组织的克林霉素含量较高,3组之间差异无显著性(P>0.05)。结论:方法准确可靠、简便、重现性好,上止血带组骨组织中克林霉素含量明显低于无止血带组,止血时机是影响克林霉素骨组织分布的主要因素。
OBJECTIVE: To establish a method for the determination of clindamycin in rabbit bone by high performance liquid chromatography (HPLC) and to study the distribution of clindamycin in the bone tissue after tourniquet. Methods: The mobile phase consisted of acetonitrile-tetrahydrofuran-phosphate buffer (30:1:70, pH 5) and the column temperature was 40 ℃. The flow rate was 0.8 mL · min-1. 250 mm × 4.6 mm, 5 μm) at a detection wavelength of 205 nm. The tourniquet was blocked at 30, 60, 90, 120 min (A, B, C, D) after intravenous clindamycin (20 mg · kg-1) Of bone tissue in the drug content. Results: Clindamycin had a good linear relationship in the range of 10.58-264.5 μg · mL-1 with RSD ≤ 2.17% and daytime RSD ≤ 2.99%. The clindamycin level in the experimental group was significantly higher than that in the control group (P <0.01). There was no significant difference (P> 0.05) between the clopidamycin group and the control group at the same time of hemostasis and different hemostatic time of 30, 60 and 90 minutes. In different hemostasis group, there was significant difference between group A and group B, C and D (P <0.01), while the clindamycin content of group B, C and D was higher than that of group B (P> 0.05). Conclusion: The method is accurate, reliable, simple and reproducible. The content of clindamycin in the cecum of the upper tourniquet group is significantly lower than that of the non-tourniquet group. The timing of hemostasis is the main factor affecting the distribution of clindamycin.