本文介绍了在高效液相色谱过程中,用荧光波长变换技术和荧光光谱对比法对环境水样中的痕量多环芳烃进行检测和鉴定以及荧光分光法用于检测和鉴别用化学方法合成得到的生物活性肽的方法。实验表明,荧光波长变换技术的应用不仅改善了检测的选择性,而且使检测灵敏度也有提高,化合物本身的荧光特性又为它自身的鉴别提供了十分有用的信息。 In this paper, the detection and identification of trace PAHs in environmental water samples by fluorescence wavelength conversion and fluorescence spectroscopy were introduced in this paper. Fluorescence spectrometry was also used to detect and identify chemically synthesized Bioactive peptides. Experiments show that the application of fluorescence wavelength conversion technology not only improves the selectivity of the detection but also increases the detection sensitivity. The fluorescence property of the compound itself provides very useful information for its own identification.