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目的:研究高压氧对模型大鼠颅脑损伤过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和核因子E2相关因子2(Nrf2)的影响。方法:健康SD大鼠30只,体重250~300 g,4~5月龄,随机分为3组:假手术组、模型组和高压氧组,每组10只,参照Feeney自由落体冲击造模法建立颅脑损伤大鼠模型。假手术组只给予手术,不造成颅脑损伤;模型组和高压氧组给予颅脑损伤处理;高压氧组用高压氧治疗,1次/d,10 d。用ELISA法检测脑组织CAT、SOD、GSH-Px和Nrf2含量,采用实时荧光定量PCR检测脑组织CAT、SOD、GSH-Px和Nrf2 mRNA相对表达量,用Western Blot测定脑组织Nrf2胞质蛋白和Nrf2核蛋白。结果:假手术组、模型组、高压氧组之间的CAT、SOD、GSH-Px、Nrf2 mRNA水平均有差异(P<0.05)。模型组脑组织CAT、SOD、GSHPx、Nrf2 mRNA水平低于假手术组(P<0.05),而高压氧组则高于模型组(P<0.05)。假手术组、模型组、高压氧组之间的脑组织CAT、SOD、GSH-Px和Nrf2核蛋白水平存在差异(P<0.05),模型组低于假手术组(P<0.05),高压氧组高于模型组(P<0.05)。Western Blot检测显示:与假手术组比较,模型组和高压氧组脑组织细胞质(核)中Nrf2蛋白表达量有明显升高(P<0.05),高压氧组升高更明显(P<0.05)。结论:高压氧对模型大鼠颅脑损伤CAT、SOD、GSH-Px和Nrf2表达具有调节作用,通过这些调节改善机体氧化性损伤。
Objective: To investigate the effects of hyperbaric oxygen on catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and nuclear factor E2 Nrf2). Methods: Thirty healthy SD rats, weighing 250-300 g and 4-5 months old, were randomly divided into three groups: sham-operation group, model group and hyperbaric oxygen group, 10 rats in each group. According to the model of Feeney free- Law to establish a rat model of brain injury. Sham operation group was given only surgery, does not cause brain injury; model group and hyperbaric oxygen group were given traumatic brain injury; hyperbaric oxygen group with hyperbaric oxygen treatment, 1 / d, 10 d. The contents of CAT, SOD, GSH-Px and Nrf2 in brain tissue were measured by ELISA. The relative expression of CAT, SOD, GSH-Px and Nrf2 mRNA in brain tissue were detected by real-time fluorescence quantitative PCR. Nrf2 nuclear protein. Results: The levels of CAT, SOD, GSH-Px and Nrf2 mRNA in sham group, model group and hyperbaric oxygen group were significantly different (P <0.05). The levels of CAT, SOD, GSHPx and Nrf2 mRNA in model group were lower than those in sham operation group (P <0.05), while those in hyperbaric oxygen group were higher than those in model group (P <0.05). The levels of CAT, SOD, GSH-Px and Nrf2 nuclear proteins in sham operation group, model group and hyperbaric oxygen group were significantly lower than those in sham operation group (P <0.05), hyperbaric oxygen Group was higher than model group (P <0.05). Western Blot showed that compared with sham operation group, the expression of Nrf2 protein in cytoplasm (nucleus) of model group and hyperbaric oxygen group was significantly increased (P <0.05), hyperbaric oxygen group was more obvious (P <0.05) . CONCLUSION: Hyperbaric oxygen can regulate the expression of CAT, SOD, GSH-Px and Nrf2 in traumatic brain injury in rats, and can improve the oxidative damage through these regulations.