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目的 探讨人粪便中 p5 3基因突变的检测用于大肠癌早期筛选诊断的可行性。 方法从 2 0例大肠癌手术切除肿瘤组织和术前粪便中分别提取DNA ,应用基因扩增 单链多态性分析 溴乙锭 (PCR SSCP EB)染色方法检测癌组织和粪便中 p5 3基因的扩增及外显因子 5～ 8的突变。 结果 2 0例大肠癌组织均有p5 35～ 8特异性扩增产物 ( 10 0 % ) ,其中有 12例发生突变 ,突变率为60 % ;2 0例粪便标本 16例有 p5 3特异性扩增产物 ( 80 % ) ,8例检测到相同的基因突变 ,其 p5 3突变检出的敏感性为 66.7% .结论 粪便中 p5 3基因突变的检测 ,具有取材方便、敏感性较高的优势 ,为大肠癌的筛选提供了一条新途径。
Objective To investigate the feasibility of the detection of p53 gene mutations in human feces for early screening and diagnosis of colorectal cancer. METHODS: DNA was extracted from 20 cases of colorectal cancer after surgical resection of tumor tissue and preoperative feces. PCR amplification of single strands was used to detect ethidium bromide (PCR SSCP EB) staining for detection of p53 gene in cancer tissues and feces. Amplification and mutation of exon 5-8. RESULTS: Twenty cases of colorectal cancer tissues had p5 35-8 specific amplification products (100%), of which 12 cases had mutations and the mutation rate was 60%; 20 cases of fecal samples had p53 specific expansion. With the increase of product (80%), the same gene mutation was detected in 8 cases, and the sensitivity of the p53 mutation was 66.7%. Conclusion The detection of p53 gene mutation in feces has the advantages of convenient material acquisition and high sensitivity. It provides a new way for the screening of colorectal cancer.