论文部分内容阅读
目的 :探讨长期面神经损伤后其运动神经元睫状神经营养因子 (CNTF)的表达及分布。方法 :以犬单侧面神经损伤为实验模型 ,采用原位杂交及免疫组织化学技术 ,结合图像分析技术测定面神经核CNTFmRNA及其蛋白表达呈阳性反应的神经元胞体灰度、体积及数量。结果 :疑核运动神经元能自分泌CNTF ,神经损伤 3周内神经元CNTF基因表达强度及反应的胞体数明显下降 ,而CNTF蛋白表达增强 ,但表达的胞体数无改变 ;4周后前两项指标迅速增高 ,而CNTF表达强度恢复正常 ,反应的胞体数无变化 ;6周后 3指标均始终低于健侧。12～ 32周各项指标均趋向稳定。结论 :长期面神经损伤面神经核仍保持较高水平的CNTF表达 ,从中枢及CNTF角度提示晚期面神经损伤仍具有修复价值
Objective: To investigate the expression and distribution of ciliary neurotrophic factor (CNTF) in motor neurons after long-term facial nerve injury. Methods: The unilateral facial nerve injury in canine was used as experimental model. The in situ hybridization and immunohistochemistry were used to detect the gray scale, volume and quantity of CNTF mRNA and protein positive neurons in facial nerve nucleus. Results: CNTF neurons were capable of autocrine of CNTF. The intensity of CNTF gene expression and the number of somatic cells decreased significantly in 3 weeks after nerve injury, while the expression of CNTF increased but the number of cells did not change. After 4 weeks, The index of the index increased rapidly, but the expression intensity of CNTF returned to normal, and the number of cell bodies did not change. After 6 weeks, the indexes of 3 were always lower than that of contralateral side. 12 to 32 weeks all the indicators are stable. CONCLUSION: CNTF remains a high level in long-term facial nerve injury. It suggests that the repair of advanced facial nerve injury from the perspective of central and CNTF