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凝血酶元时间(一期法)测定中需用组织凝血活酶,由于制备过程中存在各种影响因素,因而测得的正常值不尽相同。再由于凝血活酶悬液不易长期保存,因而每次测定时应作正常对照。在简易凝血活酶生成试验中,以稀释溶解的患者全血,作为所需全部凝血因子的来源,再与正常人基质血浆混合作用,若凝固时间超过正常3秒以上,则证明有内源性凝血因子缺陷。反之,若以稀释溶解的被试者全血与自身血浆作用,如测得的凝固时间大于正常,则证明,既可能缺乏内源性凝血因子,又可能缺乏外源性凝血因子。
Thrombin time (phase method) determination of the need for tissue thromboplastin, due to the preparation of a variety of influencing factors, which measured the normal value varies. And then because of thromboplastin suspension is not easy to long-term preservation, so each determination should be made as a normal control. In the simple thromboplastin production assay, diluted patient-dissolved whole blood was used as the source of all required clotting factors and then mixed with normal human stromal plasma. If the clotting time exceeded normal for more than 3 seconds, endogenous Clotting factor defects. Conversely, if dilution of the subject’s whole blood plasma and plasma, such as the measured coagulation time is greater than normal, then prove that both the lack of endogenous coagulation factor may also lack of exogenous coagulation factors.