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目的 :建立人类血小板抗原 1～ 4系统序列特异性引物 (PCR SSP)分型方法。方法 :合成 14条引物 ,采用PCR SSP方法对 2 5名健康献血者的HPA 1～ 4系统进行基因分型 ;分型结果与采用等位基因特异性寡核苷酸点杂交 (PCR ASO)获得的结果进行比较。结果 :以PCR SSP方法对HPA 4个系统进行分型均取得了明确、满意的结果 ,且与PCR ASO方法获得的结果完全一致。结论 :人类血小板抗原PCR SSP基因分型方法具有简便、快速、准确等优点 ,具有广泛的应用前景。
Objective: To establish a method of PCR SSP typing of human platelet antigen 1 ~ 4 sequence-specific primers. METHODS: Fourteen primers were synthesized and PCR-SSP was used to genotype HPA 1-4 of 25 healthy blood donors. The genotyping results were obtained with PCR-based dot-blot hybridization (PCR ASO) The results are compared. Results: The results of PCR SSP method were accurate and satisfactory, and the results obtained by PCR ASO method were completely consistent. Conclusion: PCR SSP genotyping of human platelet antigens has the advantages of simple, rapid and accurate, and has a wide range of applications.