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目的探讨罗格列酮(Ros)对兔急性心肌梗死(AMI)后心室重构和心功能的影响。方法将30只日本大耳白兔随机分为:假手术(Sham)组,AMI模型组,Ros组,每组10只。永久性结扎冠状动脉左前降支中上1/3处制作AMI模型,术后6hRos组给予Ros3mg/(kg·d)灌胃,AMI组及sham组给予等量蒸馏水灌胃。4周后心脏超声检查,测量左室收缩末期内径(LVESD)、左室舒张末期内径(LVEDD)、左室射血分数(EF)及短轴缩短率(FS)。用电子天平称取左心室重量(游离壁+室间隔),计算左室重量指数(左心室重量/体积质量),放射免疫法测肿瘤坏死因子α(TNF-α)变化。结果经Ros处理4周后,AMI组兔LVESD和LVEDD明显高于sham组,而EF和FS明显低于sham组(<0.01)。Ros组兔LVESD和LVEDD,左心室重量和左心室重量指数,心肌匀浆TNF-α水平显著低于AMI组(<0.01),EF显著高于AMI组(<0.01)。HE染色示AMI组心肌细胞纤维化明显,大量炎性细胞浸润,Ros组纤维化及炎性细胞浸润减少。结论 Ros抑制兔AMI后心室重构,改善心脏功能,可能与其抑制促炎症因子有关。
Objective To investigate the effect of rosiglitazone (Ros) on ventricular remodeling and cardiac function after acute myocardial infarction (AMI) in rabbits. Methods Thirty Japanese white rabbits were randomly divided into Sham group, AMI model group and Ros group, 10 rats in each group. The AMI model was made on the upper 1/3 of the left anterior descending coronary artery in the permanent ligation group, Ros3 mg / (kg · d) was given to the Ros group 6 h after operation, and the same dose of distilled water was given to the AMI group and sham group. Echocardiography was performed 4 weeks later to measure left ventricular end-systolic diameter (LVESD), left ventricular end diastolic dimension (LVEDD), left ventricular ejection fraction (EF) and short axis shortening (FS). The left ventricular mass (free wall + interventricular septum) was weighed using an electronic balance and the left ventricular mass index (left ventricular mass / weight) was calculated. The changes of tumor necrosis factor alpha (TNF-α) were measured by radioimmunoassay. Results After treated with Ros for 4 weeks, LVESD and LVEDD in AMI group were significantly higher than those in sham group, while EF and FS were significantly lower than sham group (<0.01). The LVESD and LVEDD, left ventricular mass and left ventricular mass index, TNF-α level in myocardial homogenate of Ros group were significantly lower than those of AMI group (P <0.01). HE staining showed myocardial fibrosis significantly AMI group, a large number of inflammatory cell infiltration, Ros fibrosis and inflammatory cell infiltration decreased. Conclusions Ros inhibits ventricular remodeling and improves cardiac function after AMI in rabbits, which may be related to the inhibition of proinflammatory cytokines.