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目的 :探讨鹿瓜多肽注射液治疗类风湿性关节炎 (RA)的机理。方法 :以Ⅱ型胶原 (CⅡ )诱导的免疫性关节炎 (CIA)大鼠为RA动物模型 ,通过测量其关节肿胀程度及制作病理切片并在光镜下计分 ,观察鹿瓜多肽注射液对其的治疗效果 ,并取膝关节滑膜切片进行原位杂交 ,用图像分析系统检测MMP3 和TIMP 1mRNA表达水平。结果 :与模型组比较 ,鹿瓜多肽组大鼠踝关节肿胀程度和病理损伤计分均明显减轻。模型组大鼠滑膜组织MMP3 mRNA表达阳性密度明显高于正常对照组 ,而TIMP 1mRNA表达阳性密度明显低于正常对照组。鹿瓜多肽组大鼠滑膜组织MMP3 表达降低 ,TIMP 1表达增高。结论 :鹿瓜多肽注射液对CⅡ诱导的免疫性关节炎有治疗作用 ,其机理可能与抑制滑膜组织MMP3 mRNA表达并促进TIMP 1mRNA表达有关。
Objective: To explore the mechanism of Lugua Polypeptide Injection in the treatment of rheumatoid arthritis (RA). METHODS: Rats with immune-induced arthritis (CIA) induced by type II collagen (CII) were used as animal models of RA. The degree of joint swelling was measured and pathological sections were made and scored under light microscope to observe the effect of Lugua Peptide injection on The therapeutic effect was obtained by in situ hybridization of knee joint synovial slices and the expression of MMP3 and TIMP1 mRNA was detected by image analysis system. Results :Compared with the model group, the scores of swelling and pathological lesions of the ankle joint in the Lugua Peptide group were significantly reduced. The positive density of MMP3 mRNA expression in the synovial tissue of the model group was significantly higher than that of the normal control group, and the positive expression density of TIMP 1 mRNA was significantly lower than that of the normal control group. The expression of MMP3 in the synovial tissue of deer guar polypeptide group was decreased, and TIMP-1 expression was increased. Conclusion: Lugua Polypeptide Injection has therapeutic effects on CII-induced immune arthritis. The mechanism may be related to inhibiting the expression of MMP3 mRNA in synovial tissue and promoting the expression of TIMP-1 mRNA.