论文部分内容阅读
传统的考马斯亮蓝Ｒ２５０（ＣＢＢＲ２５０）染色方法存在背景深，耗时长，试剂耗用多的缺点，考马斯亮蓝Ｇ２５０（ＣＢＢＧ２５０）虽然需时短，但其灵敏度效低。本文探讨了一步性低背景ＣＢＢＲ２５０染色方法在蛋白质ＰＡＧＥ中的应用，结果表明使用乙醇－乙酸－水染色系统，ＣＢＢＲ２５０的最佳工作浓度为０．００４‰～０．００８‰，染色时间为８～１０ｈ，灵敏度为０．５μｇ／带。一步性ＣＢＢＲ２５０染色方法与传统的ＣＢＢＲ２５０染色方法相比，有需时短，背景浅，试剂用量少的优点，可用于蛋白质的定性和定量研究。
The traditional method of staining with Coomassie Brilliant Blue R250 (CBBR250) has the disadvantages of deep background, long time consuming, and heavy reagent consumption. The Coomassie Brilliant Blue G250 (CBBG250) has short sensitivity but low sensitivity. This paper explored the application of one-step low background CBBR250 staining method in protein PAGE. The results showed that the best working concentration of CBBR250 was 0.004 ‰ ~ 0.008 ‰ and the dyeing time was 8 ~ 10h, the sensitivity of 0.5μg / band. Compared with the traditional CBBR250 staining method, the one-step CBBR250 staining method has the advantages of short time, shallow background and less reagent dosage, and can be used for qualitative and quantitative studies of proteins.