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目的:通过细胞超微结构的变化和诱导细胞凋亡的作用,来探讨瘤净片的作用机制。方法:借助外源性雌孕激素肌肉注射复制大鼠子宫肌瘤模型,通过电镜和流式细胞仪观察瘤净片对细胞超微结构和细胞凋亡的影响。结果:模型组大鼠子宫肌细胞体积增大,细胞内出现丰富的有致密小体的肌微丝,细胞器数目增多,说明肌细胞处于高度增生、活跃状态。瘤净片组大鼠子宫平滑肌细胞体积缩小,肌微丝含量较少,并见凋亡小体形成。瘤净片有明显诱导子宫平滑肌细胞凋亡的作用,细胞周期分析 DNA 直方图上见明显的凋亡峰,凋亡指数为35.51±5.81。结论:瘤净片能诱导肌瘤细胞凋亡,对子宫肌瘤有抑制作用。
OBJECTIVE: To explore the mechanism of tumor neoplasms through the changes of cell ultrastructure and induction of apoptosis. METHODS: The rat myometrium model was replicated by intramuscular injection of exogenous estrogen and progesterone. The effects of Tuomanet Tablet on ultrastructure and apoptosis were observed by electron microscopy and flow cytometry. RESULTS: In the model group, the uterine myocytes increased in size, and there were abundant microfilaments with dense bodies in the cells. The number of organelles increased, indicating that the myocytes were highly proliferating and active. The tumor volume of the uterine smooth muscle cells in the rat tumor group was reduced, and the myofilament was less, and apoptotic body formation was observed. Tuomanetian tablets obviously induced the apoptosis of uterine smooth muscle cells. Cell cycle analysis showed obvious apoptotic peaks on the DNA histogram. The apoptotic index was 35.51±5.81. Conclusion: Tuyanpian can induce apoptosis of leiomyoma cells and inhibit uterine fibroids.