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目的观察干扰素α-2b和利巴韦林联合治疗前后慢性丙型肝炎患者肝内免疫细胞动态变化情况,为研究免疫调节疗法提供依据。方法利用流式细胞计数仪对42例慢性丙型肝炎患者应用联合治疗前及其中11例治疗后患者末悄血和(或)肝脏组织进行分析。结果与对照组相比慢性丙型肝炎组肝脏内CD56~(?)、CD57~(?)、CD161~(?)细胞及CD56~(?) T淋巴细胞阳性率明显减少(P＜0．01)．CD161~(?)T淋巴细胞有减少倾向；CD56~(?)T淋巴细胞表达的CD28可见减少,CD152的表达可见增加(P＜0．05)；CD83 CD1a~(?)细胞阳性率有减少倾向,CD80~(?)CD11c~(?)、CD86~(?)CD11c~(?)细胞阳性率明显减少(P＜0．01)；显效组减低的CD56~(?)、CD161~(?)、CD56~(?)T、CD161~(?)T,CD80~(?)CD11c~(?)、CD86~(?)CD11c~(?)细胞治疗后可见增加。结论慢性丙型肝炎患者肝脏内自然杀伤细胞、自然杀伤T淋巴细胞数量及树突状细胞数量和功能减低,联合治疗使细胞免疫功能的抑制得到了改善。
Objective To observe the dynamic changes of intrahepatic immune cells in patients with chronic hepatitis C before and after treatment with interferon alfa-2b and ribavirin, and to provide basis for the study of immunomodulatory therapy. Methods Flow cytometry was used to analyze the end-stage blood and / or liver tissues of 42 chronic hepatitis C patients before and after treatment with 11 patients. Results Compared with the control group, the positive rates of CD56 (superscript +), CD57 (superscript +), CD161 (superscript +) T cells and CD56 (superscript TM) T lymphocytes in the liver of chronic hepatitis C group were significantly decreased ). CD161 ~ (?) T lymphocytes tended to reduce; CD56 ~ (?) T lymphocytes expressed CD28 decreased, CD152 expression increased (P <0.05); CD83 CD1a ~ The positive rates of CD11c ~ (?), CD86 ~ (+) CD11c ~ (~) in CD80 ~ (?) Were significantly decreased ), CD56 ~ (?) T, CD161 ~ (?) T, CD80 ~ CD11c ~ (?), CD86 ~ CD11c ~? Conclusion The number of natural killer cells, natural killer T lymphocytes and the dendritic cells in the liver of patients with chronic hepatitis C decrease. The combination therapy can improve the inhibition of cellular immune function.