为了研制南方、爪哇和花生根结线虫快速灵敏的检测和鉴定方法 ,分别分离了 4个南方根结线虫和 3个爪哇根结线虫特异性的随机扩增多态性 DNA( RAPD)片段。在这些 RAPD标记 DNA序列的基础上 ,设计了多对 SCAR PCR引物 ,并用源于国内外的南方、爪哇、花生、北方和象耳豆根结线虫群体验证其扩增特异性和灵敏度。最终确定了 3对高效扩增的SCAR引物 ,它们组合使用可以可靠灵敏地鉴定南方、爪哇和花生根结线虫。 3对引物的扩增灵敏度达 1 / 3条的二龄幼虫、雄虫或雌虫 ,这表明本研究研制的 PCR鉴定法可用于生产实践中土样和根样中 3种根结线虫快速灵敏的鉴定。 In order to develop a rapid and sensitive method for the detection and identification of root-knot nematodes in South China, Java and Peanut, four RAPD-specific DNA fragments were isolated from four M. incognita and three M. javanica. Based on these RAPD-labeled DNA sequences, a number of pairs of SCAR PCR primers were designed and their amplification specificity and sensitivity were verified by using populations of southern, java, peanut, northern and euglena root-knot nematodes originated from China and abroad. Finally, three highly amplified SCAR primers were identified, which, when used in combination, reliably and sensitively identify Southern, Java and Peanut root-knot nematodes. 3 pairs of primers amplification sensitivity of 1/3 second instar larvae, male or female, indicating that this study developed by PCR identification method can be used in the production of soil samples and root samples of three kinds of root knot nematode rapid sensitivity The identification.