论文部分内容阅读
从北京顺义和山东泰安红富士苹果园采集果实、叶片、树皮和土壤等不同基物,分离酵母菌,利用26SrDNA的D1/D2区域序列分析并结合形态学特征和SSCP分析对这些菌株进行了分类学研究,探讨了苹果园酵母的物种多样性及其分布。北京苹果园共分离酵母菌129株,鉴定为13属21种,优势属为Pichia(4个种),Cryptococcus(3个种),Pseudozyma(3个种),子囊菌占较大优势,分布于8属12种,占总种数的57.1%。山东苹果园共分离酵母291株,鉴定为13属26种,优势属为假丝酵母Candida(6个种),毕赤酵母Pichia(4个种)和隐球酵母Cryptococcus(3个种),并且子囊菌占较大优势,分布于7属17种,占总种数的65.4%。
Saccharomyces cerevisiae was collected from Shunyi, Beijing, and Fuji apple orchards in Tai’an, Shandong province. Yeasts were isolated from the leaves, barks and soils. Sequences of D1 / D2 region of 26S rDNA were used to analyze the morphological characteristics and SSCP Taxonomic study to explore apple orchard yeast species diversity and distribution. A total of 129 yeasts were isolated from apple orchards in Beijing. Among them, 21 were identified as 13 genera. The dominant genera were Pichia (4 species), Cryptococcus (3 species) and Pseudozyma (3 species) 8 genera and 12 species, accounting for 57.1% of the total species. A total of 291 yeast isolates were isolated from Shandong apple orchard, identified as 13 genera and 26 species. The dominant genera were Candida (6 species), Pichia (4 species) and Cryptococcus (3 species), and Ascomyx accounted for a greater advantage, distributed in seven genera and 17 species, accounting for 65.4% of the total species.