辣椒疫霉(Phytophthora capsici)是一种重要植物病原菌,能造成植株坏死、果实腐烂,严重影响产量[1]。辣椒疫霉侵染植物的早期病症并不明显,易被忽视。因此,对辣椒疫霉早期快速、准确检测显得尤为重要。聚合酶链式反应(PCR)为动植物病原物检测的重要方法,但需要较昂贵的仪器、试剂与耗材,后期的电泳检测也费时费力,致使这一技术很难在生产一线普及推广。Notomi等2000年研发了环介 Phytophthora capsici is an important plant pathogen, which can cause plant necrosis and fruit decay and seriously affect the yield [1]. Phytophthora capsici infected plants early symptoms are not obvious, easily overlooked. Therefore, early rapid and accurate detection of Phytophthora capsici is particularly important. Polymerase chain reaction (PCR) is an important method for the detection of animal and plant pathogens, but requires more expensive instruments, reagents and consumables. Later electrophoresis detection takes time and effort, making it difficult for this technology to popularize in the production line. Notomi et al. Developed a loop in 2000