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从山东、甘肃、青海、内蒙古、河北和黑龙江6省采集马铃薯黑痣病标本300余份,分离获得251个立枯丝核菌Rhizoctonia solani菌株。融合群测定结果表明,这些菌株分别属于多核的丝核菌AG‐3、AG1‐IB、AG4‐HG‐Ⅰ、AG4‐HG‐Ⅱ、AG4‐HG‐Ⅲ、AG‐5和AG‐11融合群。其中AG‐3是优势致病群,占分离菌株总数的71.31%;其次是AG4‐HG‐Ⅰ,占15.14%;AG‐11融合群菌株是国内首次从罹病马铃薯植株上分离得到。从各融合群中选取代表性的菌株进行5.8S rDNA‐ITS区序列分析,结果表明,隶属不同融合群或亚群菌株的5.8S rDNA‐ITS区序列存在较大的差异,而相同融合群(亚群)不同菌株的序列具有较高一致性。
More than 300 samples of potato mole nematode were collected from Shandong, Gansu, Qinghai, Inner Mongolia, Hebei and Heilongjiang provinces, and 251 strains of Rhizoctonia solani were isolated. The results of fusion assay showed that these strains belonged to the multinuclear Rhizoctonia solani AG-3, AG1-IB, AG4-HG-Ⅰ, AG4-HG-Ⅱ, AG4-HG-Ⅲ, AG-5 and AG- . AG-3 was the predominant pathogenic group, accounting for 71.31% of the total isolates; followed by AG4-HG-Ⅰ, accounting for 15.14% of the total isolates; AG-11 was first isolated from diseased potato plants in China. The 5.8S rDNA-ITS region of representative isolates from each fusant group was analyzed. The results showed that the 5.8S rDNA-ITS sequences of different fusions or subgroups had significant differences, while the same fusions Subgroups) The sequences of different strains are highly consistent.