目的探讨氧化应激在燃煤来源型氟斑牙发生中的作用。方法以高氟煤拌粘土为燃料,每天生火10 h构建高氟空气模型,取病区煤烘玉米,配制不同比例饲料喂养大鼠。复制燃煤来源型氟斑牙大鼠模型。30只SD大鼠,体重均等、雌雄各半随机分为五组:高氟空气加饲料氟含量40 mg/kg组(H组)、高氟空气加饲料氟含量25 mg/kg组(M组)、高氟空气加饲料氟含量10 mg/kg组(L组)、高氟空气加普食组(A组)及阴性对照组(C组)。饲养16周后处死大鼠,测肝、肾组织及血清中丙二醛(MDA)含量、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物(GSH-Px)活性。结果成功复制出大鼠氟斑牙(H、M组全部,L组1只);随染氟剂量的增大,差异均有统计学意义(P<0.01或P<0.05)。H、M、L组大鼠肝、血清MDA含量逐渐升高,肝、肾及血清SOD、CAT活性呈下降趋势,A组肝、肾组织仅SOD活性降低,与C组相比,GSH-Px活性变化无统计学意义。结论高氟空气可能会导致大鼠体内氧化系统与抗氧化系统的异常,在燃煤来源型氟斑牙发生过程中,空气过量氟引起的肝、肾组织氧化应激损伤可能早于其对牙体硬组织的损害,氧化应激可能是氟中毒致机体损伤和氟斑牙发生的重要机制之一。 Objective To investigate the role of oxidative stress in the occurrence of coal-derived dental fluorosis. Methods High-fluorine coal-clayey clay was used as fuel, and a high-fluorine air model was constructed after 10 h of fire. The coal was bake from corn in the ward, and different proportions of feed were used to feed rats. Copy coal-derived dental fluorosis rat model. Thirty SD rats were of equal weight and were randomly divided into five groups: Fluorine-enriched air plus feed 40 mg / kg (group H), fluoride-enriched air plus feed 25 mg / kg (group M) ), High fluoride air plus feed fluoride group 10 mg / kg (L group), high fluoride air plus general diet group (A group) and negative control group (C group). After 16 weeks of feeding, the rats were sacrificed and the contents of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH) -Px) activity. Results The dental fluorosis was successfully replicated in rats of group H and M (group L, group L). The difference was statistically significant (P <0.01 or P <0.05) with the increase of fluoride dose. The contents of MDA in liver, serum and serum of H, M and L groups increased gradually, while the activities of SOD and CAT in liver, kidney and serum decreased. The activities of SOD in liver and kidney of A group decreased. Compared with C group, GSH-Px No significant change in activity. Conclusion High fluoride air may cause abnormalities of oxidation system and antioxidant system in rats. In the process of coal-derived dental fluorosis, oxidative stress injury in liver and kidney caused by excess fluoride may be earlier than that of teeth The damage of body and hard tissue and oxidative stress may be one of the important mechanisms of organ damage and dental fluorosis caused by fluorosis.