临床观察培养的角膜干细胞羊膜移植片治疗角膜缘功能障碍的效果。方法 :人角膜缘干细胞原代培养后接种生长于羊膜上 ,临床选择角膜缘烧伤后 (碱烧伤、酸烧伤、其他化学烧伤等 )角膜新生血管的患者进行干细胞羊膜片移植 ,术后观察角膜上皮、组织浸润和新生血管情况。结果 :术后患者自觉症状轻 ,视力有一定提高 ,角膜缘移植片紧密贴附 ,角膜上皮完整 ,轻度基质炎性浸润 ,浅层新生血管减少 ,深层新生血管充盈减轻 ,睑球粘连得到松解。结论 :角膜缘干细胞羊膜片移植治疗角膜缘功能障碍能够使患者获得进一步进行角膜移植的条件 Clinical observation of cultured corneal stem cells amnion graft for the treatment of limbal dysfunction. METHODS: Human corneal limbal stem cells were primarily cultured and inoculated on the amniotic membrane. After corneal limbal burns (alkali burns, acid burns, other chemical burns, etc.) corneal neovascularization patients were selected for stem cell amniotic membrane transplantation. Corneal epithelium , Tissue infiltration and neovascularization. Results: The postoperative symptoms were mild and the visual acuity was improved. The corneal limbal graft was closely attached, the corneal epithelium was intact, the infiltration of mild stromal infiltration, the reduction of superficial neovascularization, the reduction of deep neovascular filling, the symblepharon getting loose solution. CONCLUSIONS: Limbal limbal stem cell transplantation for corneal limbal dysfunction enables patients to obtain further conditions for corneal transplantation