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应用江苏省农业科学院经济作物研究所提供的Ｂｔ转基因棉花（编号８８４０），经盆栽、田间反复鉴定对棉铃虫１龄幼虫抗虫效果平均为９３．１％，叶、蕾、铃饲喂３龄幼虫抗虫效果分别为９０％、８５％、５５％测定毒性机理。棉铃虫３龄幼虫取食抗虫棉２ｄ后，其食量、排泄量分别比该材料未导入Ｂｔ转基因对照植株减少５７．１０％，５７．１９％；幼虫长度、体重增长率分别比对照减少７１．６９％、８１．５１％。４ｄ后食量、排泄量减少７２．０２％、７７．３３％，体长和体重增长率分别比对照减少９３．０２％、１０７．７９％。测定中肠、血清ｐＨ值分别为８．１９和６．４，与对照８．３６、６．５差异不大。中肠超微结构观察，取食抗虫棉中毒死亡的棉铃虫幼虫中肠横纹肌呈收缩状；表明中肠处于中毒痉挛状态，吸收营养物质的柱状细胞微绒毛萎缩并局部被破坏，肠壁细胞质内的细胞器减少。营养物质自耗成空胞。而取食对照植株幼虫中肠超微结构则相反，肌肉舒畅。微绒毛多而正常，细胞器丰富，幼虫生长发育正常。
The Bt transgenic cotton (No. 8840) provided by Institute of Economic Crops, Jiangsu Academy of Agricultural Sciences had the average of 93.1% against the first instar larvae of cotton bollworm Insect resistance of larvae were 90%, 85%, 55% determination of toxicity mechanism. The second instar larvae of cotton bollworm fed with insect-resistant cotton two days later, their food intake and excretion decreased 57.10% and 57.19% respectively compared with those without transgenic Bt transgenic plants. The larval length and weight gain decreased by 71% .69%, 81.51%. After 4 days, the amount of food intake and excretion decreased by 72.02% and 77.33%, respectively. The body length and weight loss rate decreased by 93.02% and 107.79% respectively compared with the control. Determination of midgut, serum pH values of 8.19 and 6.4, respectively, and the control 8.36,6.5 little difference. Intestinal ultrastructure observation, cotton bollworm feeding death of cotton bollworm midgut striated muscle was shrunk; that the midgut in the poisoning spasm, absorption of nutrients in the columnar cell microvilli atrophy and partial destruction of the intestinal wall cytoplasm Organelles within the reduction. Nutrients consumable into empty cells. The feeding control larvae midgut ultrastructural contrast, muscle relaxation. More and more microvilli, rich organelles, larvae growth and development of normal.