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大豆许多重要农艺性状都是由微效多基因控制的数量性状 ,对这些数量性状进行QTL定位是大豆数量性状遗传研究领域的一个重要内容。本研究利用栽培大豆科新 3号为父本、中黄 2 0为母本杂交得到含 192个单株的F2 分离群体 ,构建了含 12 2个SSR标记、覆盖 1719 6cM、由 33个连锁群组成的连锁遗传图谱。利用复合区间作图法 ,对该群体的株高、主茎节数、单株粒重和蛋白质含量等农艺性状的调查数据进行QTL分析 ,共找到两个株高QTL ,贡献率分别为 9 15 %和 6 0 8% ;两个主茎节数QTL ,贡献率分别为 10 1%和 8 6 % ;一个蛋白质含量QTL ,贡献率为 9 8% ;一个单株粒重QTL ,贡献率为11 4 %。通过遗传作图共找到与所定位的 4个农艺性状QTL连锁的 6个SSR标记 ,这些标记可以应用于大豆种质资源的分子标记辅助选择 ,从而为大豆分子标记辅助育种提供理论依据。
Many important agronomic traits in soybean are quantitative traits controlled by micro-efficient polygenes. Mapping QTLs for these quantitative traits is an important part of genetic research on soybean quantitative traits. In this study, F2 segregation population with 192 individuals was obtained from the cross of the parent cultivar Douchyuxian 3 and Zhongzhong 20, and 12 F2 SSR markers were constructed, covering 1719 6cM and 33 linkage groups Composition of the genetic linkage map. QTL analysis of the agronomic traits such as plant height, number of main stems, grain weight per plant and protein content of the population was conducted by composite interval mapping method. Two QTLs for plant height were found, accounting for 9 15 % And 60.8% respectively. The QTL for two main stem segments contributed 10 1% and 8 6%, respectively. One QTL for protein content contributed 99.8% of the total QTL. The QTL for individual grain weight was 11 4%. Six SSR markers linked to four QTLs for agronomic traits were found by genetic mapping. These markers could be applied to the molecular marker-assisted selection of soybean germplasm resources and provide a theoretical basis for molecular marker-assisted breeding of soybean.